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工程菌体使得在感染宿主细胞时有效控制基因表达

Ting Wei^1,2, Wangsheng Lai¹, Qian Chen¹

¹CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

International journal of molecular sciences

|January 11, 2025

概括

研究人员开发了一种新的菌体感染诱导的基因表达系统,用于空间菌体辅助连续进化 (SPACE). 这种系统使特定细菌亚群的可控突变发生成为可能,推进了定向进化策略.

科学领域:

合成生物学合成生物学
分子生物学分子生物学
微生物遗传学微生物遗传学

背景情况:

空间菌体辅助连续进化 (SPACE) 需要在特定细胞亚群中进行可控的突变发生.
现有的小分子诱导系统均地影响所有细胞,限制它们在空间中的实用性.
需要精确控制细菌群体内的菌体感染引发的基因表达.

研究的目的:

开发一种菌体感染诱导的基因表达系统,用于空间中可控制的突变发生.
设计一个系统,精确地针对特定细菌亚群的菌体感染时的基因表达.
将这个系统集成到 SPACE 中,以实现菌体和宿主系统的高效定向进化.

主要方法:

工程修饰的 *Escherichia coli* (*E. coli*) 菌体冲击蛋白操作子和 *Bacillus subtilis* 西格玛因子.
开发了一种菌体感染诱导的基因表达系统,具有可调节的输出范围.
将该系统集成到 SPACE 平台中,作为一个突变发生模块.

主要成果:

通过菌体感染引发的基因表达的有效控制.
成功地将系统纳入 SPACE,促进了有针对性的进化.
进化T7RNA聚合酶突变体,具有改变的促进体识别特异性.

结论:

菌体感染诱导的基因表达系统可以精确控制细菌群体内的突变发生.
该系统是SPACE平台的宝贵补充,增强了定向进化能力.
开发的系统为人口生物学和合成生物学中的更广泛应用提供了潜力.

关键词:

指导进化是指导进化的.工程菌体的工程菌体可诱导的基因表达.菌体冲击蛋白质是一种菌体冲击蛋白质.西格玛因子 (sigma factor) 是一个因子.

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