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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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相关实验视频

Updated: Jun 3, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

基于双平面阶段检索方法的大相物体的无镜头芯片定量相位显微镜.

Yufan Chen^1,2,3, Xuejuan Wu^1,2,3, Yang Chen^1,2,3

¹Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

Sensors (Basel, Switzerland)

|January 11, 2025

概括

这项研究介绍了双平面相位检索 (BPR),这是一种在无透镜显微镜中准确的绝对相位重建的新方法. BPR克服了相解封错误,增强了细胞生物学研究的定量相成像.

关键词:

双平面的相位检索.高通量成像技术的成像代阶段检索的代阶段检索没有镜头的芯片内显微镜.定量阶段成像成像技术的使用.

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Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

相关实验视频

Last Updated: Jun 3, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

科学领域:

计算机成像成像技术
生物光子学生物光子学
细胞生物学细胞生物学

背景情况:

无透镜芯片显微镜 (LFOCM) 提供了高通量,具有成本效益的成像.
在LFOCM中代阶段检索产生了封装阶段数据 (-π到π).
阶段解封对于绝对阶段至关重要,但易发生错误的大梯度或低采样.

研究的目的:

开发一种新的方法,用于在LFOCM中准确的绝对相重建.
为了克服传统阶段解封技术的局限性.
为了提高定量相位成像 (QPI) 的准确性和稳定性.

主要方法:

提出了一种新的双平面阶段检索 (BPR) 方法.
综合相位解封的结果来自不同的传播距离.
使用HeLa和COS-7细胞活细胞成像验证了该方法.

主要成果:

BPR实现了准确的绝对相位重建.
与离轴数字全息显微镜相比,QPI精度得到了改进.
在时间间隔成像中展示了长期定量分析的稳定性和能力.

结论:

BPR有效地解决了LFOCM中的阶段解封挑战.
该方法提高了生物样本定量相位成像的可靠性.
BPR适用于长期,高通量细胞群体研究.