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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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相关实验视频

Updated: Jun 3, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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光学生物显微镜的自动光学路径对齐方法

Guojin Peng1,2, Zhenming Yu2, Xinjian Zhou2

  • 1Key Laboratory of Cognitive Radio and Information Processing, Ministry of Education, Guilin University of Electronic Technology, Guilin 541004, China.

Sensors (Basel, Switzerland)
|January 11, 2025
PubMed
概括

本研究提出了一种基于图像传感器的新方法,用于对准光学生物显微镜 (OBM),提高图像质量,而无需复杂的掩饰器. 该技术准确地确定目标,并完善对准以获得更好的性能.

关键词:
调整评估对齐的评估.生物显微镜 生物显微镜这是客观的识别.光学路径对齐的对齐.有权重的圆形配件.

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科学领域:

  • 光学工程的光学工程.
  • 生物医学成像技术技术
  • 显微镜系统 显微镜系统

背景情况:

  • 高质量的光路对齐对于光学生物显微镜 (OBM) 的优越图像质量至关重要.
  • 传统的自动调整方法通常依赖于复杂的面具检测技术.
  • 现有的方法可能缺乏低功率目标的效率和精度.

研究的目的:

  • 为低功率 (4×) 自动OBM引入一种创新的,基于图像传感器的光路线对齐方法.
  • 开发一个可靠的方法来识别和调整参考目标 (RO).
  • 为了提高超越传统技术的对齐精度和评估.

主要方法:

  • 在目标旋转过程中开发了点运动模型,以了解光学路径参数的影响.
  • 提出了使用边缘梯度和边缘位置概率的RO识别方法.
  • 引入了对齐的同心弧的基于对称的重量分布.
  • 设计了一种基于临界和位置的先进对齐评估方法.

主要成果:

  • 在不同的照明和曝光下,RO识别方法成功地区分了4×和10×目标.
  • 重量循环装配方法实现了低于1.16%的误差半径比.
  • 精细调整阶段表现出更利的评估曲线.
  • 调整估计方法在和环境中显示了0.875像素的平均误差.

结论:

  • 拟议的基于图像传感器的方法为OBM光路线对齐提供了有效和精确的替代方案.
  • 新的RO识别和对齐评估技术提高了准确性和稳定性.
  • 这种方法适用于低功率的自动OBM,提高了系统的整体性能.