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抑制植物衍生的水平转移基因BtSC5DL有效控制了Bemisia tabaci MED的发生

Yifan Liu^1,2, Cheng Gong^2,3, Yuan Hu²

¹College of Plant Protection, Hunan Agricultural University, Changsha, China.

Pest management science

|January 11, 2025

概括

白 (Bemisia tabaci) 使用一种植物衍生的基因,BtSC5DL,用于胆固醇合成,增强其繁殖能力. 沉默这个基因可以减少白种群,提供一种新的害虫控制策略.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
虫害管理虫害管理虫害管理

背景情况:

白 (Bemisia tabaci) 是一种主要的农业害虫,会破坏作物并传播病毒.
白的适应性和繁殖能力由水平转移的基因 (HTG) 增强,包括那些参与胆固醇合成的基因.
一个来自植物的基因,B. tabaci Δ7-sterol C5-desaturase-like (BtSC5DL),被确定为白胆固醇代谢和繁殖的关键.

研究的目的:

调查植物衍生BtSC5DL基因在地中海白草 (MED) 中的作用.
评估BtSC5DL对白胆固醇代谢和生殖能力的影响.
评估针对BtSC5DL进行白害虫控制的潜力.

主要方法:

来自B. tabaci MED. 的BtSC5DL基因的克隆和鉴定.
生物信息学和分子分析以确认基因的起源和表达.
使用dsRNA养和病毒诱导的基因沉默 (VIGS) 来评估功能影响的基因沉默.

主要成果:

来自植物的BtSC5DL稳定地集成并表达在B. tabaci. 中.
沉默BtSC5DL显著降低了白的卵产量和胆固醇水平.
通过VIGS对BtSC5DL的长期抑制表明有效控制白种群.

结论:

BtSC5DL基因对于胆固醇生物合成至关重要,并增强了B. tabaci MED. 的繁殖成功.
针对BtSC5DL提供了一个有前途的基于RNA干扰 (RNAi) 的策略,用于管理白害虫.
这项研究为开发新的,可持续的农业害虫控制方法提供了基础.

关键词:

贝米西亚烟草 (Bemisia tabaci) 是一种烟草.这是一种RNA干扰.横向基因转移是指水平基因转移.害虫控制害虫控制害虫控制害虫控制固醇代谢的代谢过程

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