循环Dof因子3通过激活Rosa roxburghii水果中的GDP-l-银酸酶来调解依赖光的酸盐生物合成
Qianmin Huang1, Yali Yan1, Xue Zhang1
1Guizhou Engineering Research Center for Fruit Crops, Agricultural College, Guizhou University, Guiyang, Guizhou, China.
Plant physiology
|January 11, 2025
概括
在植物中,光诱导的l-ascorbate (AsA) 生物合成是由RrHY5-RrCDF3-RrGGP2模块调节的. 这一途径通过控制对光信号的响应关键基因表达来增强Rosa roxburghii中的AsA产生.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生化学
- 植物生理学 植物生理学
背景情况:
- 光线通过基因调节显著影响植物中的l-ascorbate (AsA) 水平.
- 驱动光依赖的AsA生物合成的精确转录机制尚未完全理解.
研究的目的:
- 为了阐明在Rosa roxburghii中光诱导的AsA生物合成的分子机制.
- 在光照条件下识别参与调节AsA代谢基因的转录因子.
主要方法:
- 对RrGGP2基因的促进体分析,以识别对光敏感的动机.
- 酵母单杂交和双化酶测定以确认转录因子的结合和活性.
- 在R. roxburghii和Solanum lycopersicum的暂时和稳定的过度表达/沉默实验中.
主要成果:
- 循环Dof因子3 (RrCDF3) 被确定为一种对光敏感的转录因子,激活RrGGP2表达.
- RrCDF3直接与RrGGP2促进体结合,调节AsA合成.
- 延长型HYPOCOTYL5 (RrHY5) 通过与其促进体相互作用来增强RrCDF3转录.
- 过度表达RrCDF3增加了R. roxburghii和S. lycopersicum中的AsA含量.
结论:
- 一个新的光介导调节通路,RrHY5-RrCDF3-RrGGP2模块,控制了R. roxburghii果实中的AsA生物合成.
- 这条通路为光信号提供了一种机制,以调节AsA的产生,并有可能用于提高作物中维生素C的含量.
相关概念视频
01:46The Calvin Benson Cycle
4.4K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.4K
01:40The Calvin Cycle
73.5K
Overview
73.5K
01:28The Citric Acid Cycle: Output
7.6K
The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
7.6K
02:36The Citric Acid Cycle
150.8K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
150.8K
01:27C4 Pathway and CAM
45.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.2K
01:37The Citric Acid Cycle: Overview
16.0K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
16.0K