通过CHROMATIN REMODELING 11调解的染色质可访问性可以促进大米的冷却耐受性
Jing Li1, He Liu2, Hanying Qian1
1The State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China.
米染色体改造11 (OsCHR11) 通过增加染色体可访问性和基因表达,增强耐寒性. 失去OsCHR11功能会降低大米植物的冷却耐受性.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 染色体重塑对于基因转录至关重要.
- 它通过染色质可访问性对植物耐寒性的作用尚未得到研究.
研究的目的:
- 研究大米染色体改造11 (OsCHR11) 在冷却耐受性方面的功能.
- 阐明OsCHR11用来调节冷反应的机制.
主要方法:
- 产生了OsCHR11.1的功能丧失突变体.
- 进行了转录组和ATAC-seq分析.
- 使用了ChIP-seq和多omics集成.
主要成果:
- OsCHR11突变显示出增加的冷却易感性和受损的转录基因反应.
- OsCHR11增强了对冷反应基因促进者的染色质可访问性.
- OsCHR11对于表达关键的冷反应基因至关重要,包括在三糖生物合成中的基因.
结论:
- OsCHR11积极调节大米的冷却耐受性.
- OsCHR11通过增加染色质可访问性和基因表达而起作用.
- 三糖的生物合成部分调解了OsCHR11的耐寒效应.
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