相关实验视频

Updated: Jun 3, 2025

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

通过CRISPR检测心脏瘤相关的microRNAs

Youlin Fu¹, Peng Zhang¹, Feng Chen¹

¹Department of Cardiology, Ganzhou People's Hospital, Ganzhou, Jiangxi, China.

Molecular biology reports

|January 11, 2025

概括

基于CRISPR的诊断 (CRISPR-Dx) 提供了一种有希望的分子方法,通过识别微RNA (miRNA) 失调来检测罕见的心脏瘤. 本综述探讨了CRISPR-Dx系统用于心脏瘤诊断和预后中的miRNA检测.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
在瘤学瘤学.

背景情况:

心脏瘤很难仅仅通过成像来诊断.
微RNA (miRNA) 失调与心脏瘤,如心房肌瘤和血管肉瘤有关.
分子诊断为罕见的心脏瘤提供了替代检测方法.

研究的目的:

对微RNA (miRNA) 检测的基于CRISPR的诊断 (CRISPR-Dx) 进行批判性审查.
专注于CRISPR-Dx在诊断和确定心脏瘤的预后中的应用.
讨论CRISPR-Dx在这个领域的进展,挑战和未来方向.

主要方法:

通过Cas蛋白 (Cas9,Cas12,Cas13,Cas12f) 分类的多种CRISPR-Dx系统的概述.
讨论CRISPR/Cas系统与同热放大集成,用于低丰度miRNA检测.
包括无放大CRISPR-Dx系统用于直接检测miRNA.

主要成果:

克里斯普尔-Dx系统能够进行视觉读取,便携性和快速的多重miRNA检测.
已经开发了各种CRISPR-Dx平台,有和没有放大,用于miRNA分析.
突出了CRISPR-Dx在敏感和特定检测心脏瘤相关的miRNAs方面的潜力.

结论:

CRISPR-Dx代表了罕见心脏瘤分子诊断的重大进展.
需要进一步的研究和开发来克服当前的陷,并优化CRISPR-Dx在心脏瘤学中的临床应用.
通过miRNA检测,CRISPR-Dx有望改善心脏瘤的诊断和预后.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?心脏癌症是什么心脏癌症是什么一个心脏瘤.这是心脏癌症,心脏癌症.POCT POCT 在线观看这是一个微型RNA.

更多相关视频

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...