相关实验视频
Updated: Jun 3, 2025
07:48
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
12.4K
抑制膀癌的进展使用基于网络药理和生物信息学方法的环旋素D
Ke Gao1, Hanbing Jiang2, Chao Zhang2
1Department of Urology, Affiliated Xi'an Peoples Hospital (Xi'an Fourth Hospital) of Northwest University, Xi'an, 710000, China.
Naunyn-Schmiedeberg's archives of pharmacology
|January 11, 2025
概括
循环维洛布素D (CVB-D) 通过抑制癌细胞生长和诱导亡,在治疗膀癌 (BCa) 中表现有前途. 这项研究确定GSN是BCa治疗中CVB-D的关键治疗标.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 膀癌 (BCa) 的治疗选择有限,死亡率高.
- 环旋素D (CVB-D) 是一种天然的类化合物,显示出抗瘤的潜力.
- 在BCa中,CVB-D的特定效应和分子标尚未得到充分理解.
研究的目的:
- 为了研究CVB-D对膀癌细胞系的疗效.
- 确定参与CVB-D抗BCa作用的分子标和信号通路.
- 探索BCa治疗中已识别的点的临床相关性.
主要方法:
- 在体外测试 (CCK8,殖民地形成,流细胞计) 来评估CVB-D对BCa细胞的影响.
- 生物信息分析 (PPI网络,CytoHubba,GO,KEGG) 以确定治疗点和途径.
- 分子对接,分子动力学,RT-qPCR和西方抹杀以验证GSN作为目标.
主要成果:
- CVB-D显著抑制了BCa细胞的增殖和诱导了细胞亡.
- 确定了COL1A1,COL6A1,COL6A2,COL5A2,COL5A1,COL12A1,COL18A1,ITGA5,VCL,FLNA和GSN作为主要目标.这些目标包括:
- PI3K/AKT通路与CVB-D的抗癌机制有关.
- 确定GSN是最有效的目标,CVB-D抑制其表达.
结论:
- 通过抑制增殖和诱导亡,CVB-D表现出显著的抗BCa活性.
- 在膀癌治疗中,GSN是CVB-D的关键分子标.
- 这项研究为在BCa治疗中使用CVB-D提供了理论基础.
相关概念视频
02:34Inhibition of Cdk Activity
4.6K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.6K
02:57Targeted Cancer Therapies
7.4K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.4K
02:49Cancer Therapies
7.5K
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
7.5K
02:50Combination Therapies and Personalized Medicine
4.8K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
4.8K
01:20The Retinoblastoma Gene
4.0K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.0K
01:45Positive Regulator Molecules
105.7K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
105.7K