在过敏性鼻炎中Piezo1-诱导的鼻上皮质屏障功能障碍
Shengyang Liu1,2, Jianhua Wu1,3, Linghui Meng1
1Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Duanxing West Road, Jinan, 250000, Shandong, China.
Inflammation
|January 11, 2025
概括
皮埃佐1通道在过敏性鼻炎 (AR) 中升高,导致鼻障碍功能障碍. 减少人鼻细胞中的Piezo1表达恢复了屏障完整性,这表明它是AR的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 过敏性鼻炎 (AR) 涉及鼻上皮质屏障功能障碍.
- 皮埃佐1在AR病变发生过程中的作用尚不清楚.
研究的目的:
- 调查Piezo1在AR鼻上皮质屏障功能障碍中的作用.
- 探索Piezo1对紧密交叉点和ERK1/2通路的影响.
主要方法:
- 收集了人类鼻腔粘膜样本,并使用了AR的小鼠模型.
- 用人鼻上皮细胞 (HNEpCs) 进行体外研究.
- 通过RT-PCR,免疫光学和西式涂抹评估了Piezo1,ERK1/2和紧结 (TJ) 组件表达.
主要成果:
- 在AR患者和AR小鼠中Piezo1表达升高.
- 在AR中减少TJ成分 (ZO-1,奥克卢丁,克劳丁-1) 的表达.
- 在HNEpC中Piezo1的淘汰恢复了TJ水平,并改善了屏障完整性.
- 观察到Piezo1和ERK1/2表达之间的负相关性.
结论:
- 在AR中,Piezo1对于鼻上皮质屏障功能障碍至关重要.
- 在AR中,Piezo1调节TJs和ERK1/2通路.
- Piezo1代表了过敏性鼻炎的潜在治疗标.
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