相关实验视频

Updated: Jun 3, 2025

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

19.7K

通过抗体识别生物化来识别内源近位蛋白的协议.

Camilla Rega1, Mercedes Pardo1, Lesley-Ann Martin1

  • 1The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.

STAR protocols
|January 11, 2025
PubMed
概括

通过抗体识别 (BAR) 生物化提供了一种用于绘制细胞内的蛋白相互作用的新方法. 这种基于抗体的技术可以识别接近目标的蛋白质,而不需要基因工程.

关键词:
细胞生物学 细胞生物学蛋白质生物化学 蛋白质生物化学蛋白质组学 蛋白质组学

更多相关视频

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks07:02

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Published on: May 17, 2020

24.0K
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Published on: July 3, 2017

9.5K

相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K