使用单细胞细胞毒性测定评估免疫细胞相互作用的协议

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Updated: Jun 3, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

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Zhihao Wei1, Konglan Lin1, Min Huang1

  • 1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China; Breast Tumor Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.

STAR protocols
|January 11, 2025
PubMed
概括

这项研究引入了一种使用微波阵列和自动显微镜的新方法,用于实时观察自然杀手 (NK) 细胞细胞毒性. 该技术允许对免疫细胞相互作用及其动态细胞毒性过程进行详细的单细胞水平分析.

关键词:
癌症 癌症 癌症 癌症基于细胞的测试临床协议 临床协议免疫学 免疫学 免疫学

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Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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