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相关概念视频

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

841
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
841

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相关实验视频

Updated: Jun 3, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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使用单细胞细胞毒性测定评估免疫细胞相互作用的协议.

Zhihao Wei1, Konglan Lin1, Min Huang1

  • 1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China; Breast Tumor Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.

STAR protocols
|January 11, 2025
PubMed
概括

这项研究引入了一种使用微波阵列和自动显微镜的新方法,用于实时观察自然杀手 (NK) 细胞细胞毒性. 该技术允许对免疫细胞相互作用及其动态细胞毒性过程进行详细的单细胞水平分析.

关键词:
癌症 癌症 癌症 癌症基于细胞的测试临床协议 临床协议免疫学 免疫学 免疫学

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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
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相关实验视频

Last Updated: Jun 3, 2025

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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 标准的流式细胞计测试检测细胞毒性缺乏动态过程监测.
  • 实时观察免疫效应细胞-标细胞相互作用是具有挑战性的.

研究的目的:

  • 提出一种持续观察自然杀手 (NK) 细胞介导的细胞毒性的方案.
  • 为了使动态免疫-细胞相互作用的单细胞水平分析.

主要方法:

  • 使用微波阵列与自动化显微镜进行连续监控.
  • 主要NK细胞的隔离和标记.
  • 将单元格加载到微波阵列和图像分析.

主要成果:

  • 该协议有助于持续观察NK细胞介导的细胞毒性.
  • 能够在单细胞水平上对免疫细胞相互作用进行详细分析.

结论:

  • 该协议为研究细胞毒性的动态提供了一种新的方法.
  • 为了解NK细胞功能和免疫反应提供了有价值的工具.