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准备,修改,表征和稳定性评价5甲基四基酸脂体的5-甲基四基酸脂体

Ziyi Meng¹, Xu Yan², Nima Azarakhsh³

¹Department of Food Science and Technology, Jinan University, Guangzhou 510632, China; China Mengniu Dairy Co., Ltd, Hohhot 011517, China.

|January 11, 2025

概括

这项研究开发了一种新型的pectin-chitosan nanoliposome (P-CH-NL),以改善5-methyltetrahydrofolate (5-MTHF) 的稳定性和消化,这是一种优质的叶酸补充剂. 该P-CH-NL配方表现出增强的5-MTHF保留和有针对性的交付,以提高生物可用性.

科学领域:

营养生物化学营养生物化学
材料科学材料科学材料科学
药物输送系统药物输送系统

背景情况:

叶酸是一种必不可少的维生素B,对单碳代谢至关重要.
作为补充剂,5-甲基四基叶酸 (5-MTHF) 与叶酸和食品叶酸相比具有优势.
提高5-MTHF的稳定性和消化性是有效补充的关键.

研究的目的:

开发一种稳定且可消化的5-甲基四基酸盐 (5-MTHF) 配方.
为了利用一层一层的自我组装方法来进行纳米脂质体修饰.
为了评估5MTHF输送的pectin-chitosan nanoliposomes (P-CH-NL) 的性能.

主要方法:

通过逐层自我组装,对纳米脂质体 (NL) 用奇托 (CH) 和点 (P) 进行修改.
创建核心结构化奇多-纳米酶体 (CH-NL) 和点-奇多-纳米酶体 (P-CH-NL).
评估封装效率,加载效率,颗粒大小,物理稳定性和体外消化.

主要成果:

P-CH-NL的封装效率为64.62%,加载效率为1.05 mg/g,粒子大小为285.86 nm.
在各种压力条件下 (盐,pH,氧化,热,发酵,紫外线) P-CH-NL表现出优越的物理稳定性和高的5-MTHF保留率 (>80%).
在体外消化研究证实P-CH-NL保护了5-MTHF释放到小肠.

结论:

多层脂质体加载的5-MTHF,特别是P-CH-NL,为稳定且易消化的叶酸补充提供了一个有希望的方法.
开发的纳米脂质体系统增强了5-MTHF的生物可用性和治疗潜力.
与传统形式相比,这种技术为叶酸补充提供了更好的替代方案.

关键词:

5甲基四基叶酸 5-甲基四基叶酸可消化性可消化性一层一层的自组装.纳米脂质体中的纳米脂质体稳定的稳定性稳定的稳定性

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