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相关概念视频

Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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干素分离物用于开发用于质检测的参考材料.

Majlinda Xhaferaj1, Gabriella Muskovics2, Zsuzsanna Bugyi3

  • 1Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Bioactive and Functional Food Chemistry, Karlsruhe, Germany.

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概括

开发精确的质检测对于乳病患者至关重要. 这项研究创建了麦质分离物作为标准,改进了与麦污染的无质产品中的质分析.

关键词:
结核病是什么? 结核病是什么?与酶相关的免疫吸收试验 (ELISA)液体染色学并联质谱学 (LC-MS/MS) 是一种流体染色学.逆相高性能液态染色学 (RP-HPLC) 是一种它们是Secalins和Secalins.二甲基硫酸盐聚烯胺凝电泳 (SDS-PAGE) 的方法

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科学领域:

  • 食品科学 食品科学 食品科学
  • 分析化学是一种分析化学.
  • 胆固醇疾病研究研究.

背景情况:

  • 无质产品需要<20 mg/kg的质,以确保乳安全.
  • 目前的分析方法缺乏麦特定的参考材料.
  • 无质食品中的玉米污染构成了分析挑战.

研究的目的:

  • 调查收获年对麦质成分的影响.
  • 开发独特的麦蛋白分离物,用于校准标准.
  • 为了提高含粮食品中质检测的准确性.

主要方法:

  • 采用四种不同的方法提取麦蛋白:前胺 (PROL),谷蛋白 (GLUT),谷蛋白 (G) 和乙尼/水可提取蛋白 (AWEP).
  • 通过RP-HPLC,GP-HPLC,SDS-PAGE和LC-MS/MS对分离物的表征.
  • 使用R5 ELISA对分离物的评估,以确定它们的反应顺序.

主要成果:

  • 成功生成了四种不同的麦蛋白分离物.
  • 隔离物体的表征证实了它们独特的组成.
  • R5 ELISA 显示了反应顺序:PROLiso > AWEPiso > Giso > GLUTiso.

结论:

  • 这项研究为质分析提供了必要的麦特异性参考材料.
  • 开发的分离物对于准确量化麦污染食品中的质至关重要.
  • 这些发现代表了在确保无质产品安全方面取得的重大进展.