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南极油中的毒性及其对人类肠道细胞的影响

Haiyan Zhang¹, Yunyun Ji¹, Zhongquan Jiang²

¹East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.

Ecotoxicology and environmental safety

|January 11, 2025

概括

南极油 (AKO) 中的会通过增加氧化应激和亡来引起细胞损伤. 矿是最有毒的物种,影响了Caco-2细胞活力和信号通路.

科学领域:

环境毒理学环境毒理学
细胞生物学细胞生物学
食品安全科学食品安全科学

背景情况:

在南极油 (AKO) 中生物积聚,对人类健康构成潜在风险.
AKO含有不同毒性的各种物种.

研究的目的:

在AKO中使用Caco-2细胞调查毒性.
确定对氧化应激和亡的影响.

主要方法:

暴露Caco-2细胞的标准和AKO.
评估了细胞活力,LDH释放,氧化应激标志物 (SOD,CAT,MDA,GSH-Px),ROS和亡.
分析了与亡相关的基因和PI3K/AKT/mTOR通路.

主要成果:

化物 (As3+) 是最有毒的物种,其次是化物 (As5+),DMA和AsB.
在24小时后,高AKO度将细胞活力降低到63.95%.
暴露增加了ROS,破坏了线粒体潜能,调高了亡基因 (Caspase-3, -9,Bax) 和调低了PI3K/AKT/mTOR信号传递.

结论:

在AKO中的会通过氧化应激和Caco-2细胞的亡诱导细胞毒性.
调查结果强调需要对AKO产品进行严格的食品安全评估.

关键词:

南极油油南极油油细胞灭亡 (apoptosis) 是一种死亡的过程.的物种的物种在Caco-2细胞中.氧化应激是一种氧化应激.

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