具有临床意义的抗微生物耐药性肠道细菌在沉默的天中
Aneta Kovarova1, Graham Prole2, Maeve Louise Farrell1
1Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, University of Galway, Ireland; Centre for One Health, University of Galway, Ireland.
The Science of the total environment
|January 11, 2025
概括
像无声天这样的城市水鸟携带重要的耐药细菌和抗菌素耐药性基因 (ARG). 这些发现突出了天作为监测野生动物环境中的抗菌素耐药性 (AMR) 的潜在哨兵.
科学领域:
- 环境微生物学环境微生物学
- 一个健康,一个健康.
- 抗微生物耐药性 (AMR) 监测监测
背景情况:
- 城市水体从废水中接收抗微生物和耐药细菌.
- 这些环境中的野生动物可以获得和传播具有临床意义的抗微生物耐药性基因 (ARG).
研究的目的:
- 为了评估临床上重要的耐药细菌在城市无声天中的患病率.
- 在这些水禽中识别特定的耐药机制和细菌克隆.
主要方法:
- 从无声天的便样本中培养出耐药细菌 (耐碳烯,产生ESBL,耐西,耐胆固醇).
- 使用MALDI-TOF确定了分离物,并进行了抗微生物敏感性测试.
- 实时PCR检测到特定的耐药基因 (例如CTX-M,CRGs,mcr).
- 全基因组测序 (WGS) 鉴定了耐药分离物,包括碳烯酶和mcr基因生产者.
主要成果:
- 65%的天样本产生了耐药生物体,主要是大肠杆菌 (82%).
- 20个大肠杆菌分离物产生扩展谱β-乳糖酶 (ESBL),CTX-M-15是最常见的.
- 检测到碳烯耐药性,包括blaOXA-181和blaCMY-141基因.
- 一个分离物携带移动胆固醇抗性 (mcr-1) 基因.
- 确定了高风险的致病性ESBL大肠杆菌克隆 (ST38,ST69,ST131).
结论:
- 沉默的天作为临床相关的耐药肠杆菌和ARG的储存库.
- 这些发现表明,沉默的天可以作为一个有价值的哨兵物种,在城市野生动物中监测AMR.
- 了解城市生态系统中的AMR传播途径对于公共卫生至关重要.
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