相关实验视频

Updated: Jun 14, 2026

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Published on: November 19, 2009

为了测量Bacillus subtilis中绝对膜潜力的测量,使用光寿命

Debjit Roy¹, Xavier Michalet², Evan W Miller³

¹UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California at Los Angeles, Los Angeles, California; Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California.

Biophysical reports

|January 11, 2025

概括

研究人员开发了一种新的光寿命方法,用于精确测量细菌膜潜力. 这项技术量化了单个细菌细胞中的膜潜力,揭示了生理多样性.

科学领域:

细菌电生理学细菌电生理学
微生物生理学微生物生理学
生物物理技术生物物理技术

背景情况:

细菌膜潜力 (MP) 反映了细胞功能,新陈代谢和压力.
现有的光学方法对于细菌的绝对MP测量缺乏精度.
传统的电极方法不适合小细菌细胞.

研究的目的:

开发一种新的,校准的光学方法,用于细菌中精确的单细胞MP测量.
量化MP分布和细菌群的变化.
建立一个细菌生物电力研究的基础.

主要方法:

使用基于光寿命的方法,使用VoltageFluor (VF) 光传感器.
采用定量相位-FLIM分析用于高通量MP读出.
使用调制的梯度和Bacillus subtilis中的离子体校准了MP.

主要成果:

实现了单细胞解析的MP分布和扰动时的变化.
建立了针对Bacillus subtilis.特定的基于VF生命周期的MP校准.
估计的不扰乱的MP值 (MSgg中的-65 mV,M9中的-127 mV) 和去极化MP (MSgg中的-14 mV).
揭示了人口层面的MP异质性 (6-10mV),表明生理多样性.

结论:

这种新的光生命周期方法可以在细菌中准确,定量地测量MP.
该技术提供了细菌生理和代谢异质性的见解.
这项工作促进了细菌电生理学和生物电力的研究.

更多相关视频

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

相关概念视频

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...