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祖先的碳酸无水酶具有显著增强的稳定性和活性,用于CO2捕获和利用

Chun Yang¹, Lei Mao¹, Yaxin Chen¹

¹Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China.

Bioresource technology

|January 11, 2025

概括

改造的祖先碳酸无水酶 (CA) 在碳捕获方面表现出了显著的稳定性. 这种新酶AncCA19在恶劣条件下保持高活性,非常适合碳捕获,利用和储存 (CCUS).

科学领域:

生物化学生化学
酶工程是什么? 酶工程是什么?
环境科学环境科学

背景情况:

碳酸 (CA) 对于二氧化碳捕获至关重要,但在工业条件下往往不稳定.
现有的CAs在碳捕获,利用和储存 (CCUS) 应用的高温和高压下迅速失效.

研究的目的:

为高效可靠的碳捕获设计一种高度稳定的碳酸无水酶.
利用祖先序列重建 (ASR) 来创建一个强大的CA酶.

主要方法:

采用了优化祖先序列重建 (ASR) 方法,使用可靠的数据集来设计新的CA.
预先选了潜在的候选人,以确定最有前途的祖先,AncCA19.
在各种条件下表达和描述AncCA19的水合活性,最佳温度和pH值以及稳定性.

主要成果:

改造的酶AncCA19表现出异常的水化活性 (58,859 WAU/mg).
在100°C和pH9下,AncCA19表现最佳,其半衰期在95°C为1.7小时.
在人造海水 (47,370 WAU/mg) 和N-甲基二甲醇胺 (MDEA) (6,596 WAU/mg) 中长时间化后,该酶保持了显著的活性,表明其高稳定性.

结论:

在稳定性和性能方面,AncCA19代表了碳酸无水化合物的新基准.
这种工程 CA 是一个多功能和有效的候选人,用于各种 CCUS 应用,需要强大的酶.
通过ASR方法,成功地产生了一种高度稳定的酶,适合于具有挑战性的工业环境.

关键词:

祖先序列重建的重建.酶性CO2转化转化酶工程是什么?酶工程是什么?热活性酶是一种热活性酶.

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