非传统酵母:有机酸生物生产的有前途的细胞工厂
Yupeng Wang1, Yi Wang1, Jiakai Cui2
1Department of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Trends in biotechnology
|January 11, 2025
概括
非传统的酵母在低pH值下提供强大的有机酸生产,简化了下游加工. 基因组工程的进步提高了它们的代谢能力,用于工业应用.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 合成生物学 合成生物学
背景情况:
- 微生物有机酸生产受到低酸耐受性和高盐再加工成本的限制.
- 非传统的微生物表现出环境适应性,使得在低pH条件下能够产生酸.
- 这简化了下游加工,并降低了整体生产成本.
研究的目的:
- 审查用于有机酸生产的非传统酵母的环境适应性.
- 讨论最近的基因组工程策略,用于代谢工程这些酵母.
- 要突出生产C3,C4和C6有机酸的持续努力和挑战.
主要方法:
- 文献综述侧重于非传统酵母的适应性.
- 对基因组工程技术进行分析,以操纵代谢途径.
- 使用非传统酵母生产C3,C4和C6有机酸的案例研究.
主要成果:
- 非传统的酵母在酸性环境中表现出显著的强度.
- 基因组工程工具已经推进了这些生物体的代谢工程.
- 对于生产各种有机酸的成功例子存在,尽管仍然存在挑战.
结论:
- 非传统的酵母是强大的有机酸生物合成的有希望的平台.
- 在代谢工程的进一步发展可以优化生产产量和效率.
- 本综述为创建优质有机酸生产微生物菌株提供了指导.
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