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Updated: Jun 3, 2025

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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克里斯普尔混合:一种克里斯普尔介导的细胞内定向进化平台,用于RNA适应体

Qiwen Su-Tobon1, Jiayi Fan1, Michael Goldstein1

  • 1Department of Chemistry, Boston College, Chestnut Hill, MA, USA.

Nature communications
|January 11, 2025
PubMed
概括

研究人员开发了一种新的CRISPR系统,以进化RNA吸收体,以精确调节基因. 这种方法使哺乳动物细胞中的多个基因能够同时激活和抑制,使用正交的阿普坦-RNA结合蛋白对.

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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