在标准的谷氨基基基板上,III类谷氨素ROXY9的酶性无活性的分子基础
Pascal Mrozek1, Stephan Grunewald1, Katrin Treffon1
1Department of Plant Molecular Biology and Physiology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, Julia-Lermontowa-Weg 3, 37077, Göttingen, Germany.
Nature communications
|January 11, 2025
概括
植物中的III类谷氨素ROXY9在酶上是不活性的,与I类GRX不同. 其活性部位的结构变化阻止了谷氨的结合,这表明这种植物特异性蛋白质具有独特的调节作用.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 蛋白质的结构和功能.
背景情况:
- 一类谷氨基素 (GRXs) 是必要的酶,可催化谷氨基依赖的减少.
- 陆地植物拥有独特的III类GRX家族,包括ROXY9,它通过未知的机制调节TGA转录因子.
研究的目的:
- 为了研究Arabidopsis thalianaIII类GRX ROXY9.9.的酶活性和谷氨结合特性.
- 阐明ROXY9与I类GRX相比具有潜在的独特功能的结构基础.
主要方法:
- 使用模型基质进行酶分析.
- 在不同的谷氨二硫化物 (GSSG) /谷氨 (GSH) 氧化还原潜力下分析谷氨化.
- 活性部位和GSH结合部位的结构分析.
主要成果:
- ROXY9在常见基质上没有氧化还原酶活性.
- ROXY9的活性位点谷氨基化需要高度氧化的GSH/GSSG条件,与I类GRX不同.
- 在ROXY9的GSH结合部位的结构变化可能会阻碍GSH结合,并解释其酶性不活性.
结论:
- ROXY9的酶性不活性归因于其活性部位的结构差异,防止有效的GSH结合.
- 这种不活动可能代表了一个进化策略,以防止与I类GRXs的功能重叠.
- 需要进一步的研究来确定ROXY9是否通过不同于酶活性的氧化还原机制调节TGA转录因子.
更多相关视频
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10.0K
07:47Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
2.8K
相关概念视频
01:30The Electron Transport Chain
16.0K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.0K
01:17Oxidation of Phenols to Quinones
2.9K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
2.9K
01:43Electron Transport Chain: Complex III and IV
7.0K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.0K
02:34Enzymes
80.6K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
80.6K
02:32Exon Recombination
3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.5K
02:47Ribozymes
11.2K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
Ribozymes can...
11.2K