相关实验视频

Updated: Jun 3, 2025

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Published on: August 31, 2017

使用Myotally软件量化小鼠骨肌肉截面的肌肉纤维大小,数量和中心核的协议

Pieter Both¹, Soochi Kim², Jengmin Kang³

¹Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Stem Cell Biology and Regenerative Medicine Graduate Program, Stanford University School of Medicine, Stanford, CA 94305, USA.

|January 12, 2025

概括

这项研究介绍了Myotally,这是一款用于自动分析小鼠骨肌肉的用户友好的软件. 它有效地从免疫光图像中量化肌肉纤维特征,提高了研究的一致性.

科学领域:

生物医学工程生物医学工程
肌肉生理学肌肉生理学
计算生物学计算生物学

背景情况:

准确量化肌肉纤维特征对于理解肌肉疾病至关重要.
手动分析肌肉截面是耗时的,容易变化.
需要自动化工具来提高肌肉研究的效率和可重复性.

研究的目的:

介绍使用Myotally软件进行肌肉纤维特性自动分析的协议.
为了证明软件在量化肌肉纤维大小,数量和中央核化的能力.
突出Myotally在简化肌肉研究工作流程方面的潜力.

主要方法:

开发了一个名为Myotally的用户友好的软件,用于自动化图像分析.
协议包括软件安装,图像准备和参数选择.
使用免疫光染色小鼠骨肌肉截面进行分析.

主要成果:

Myotally能够快速和自动量化肌肉纤维大小,数量和核心核的量化.
该软件允许选择关键参数,包括图像质量和尺寸限制.
可选功能,如平均光度测量,是可用的.

结论:

Myotally显著提高了肌肉纤维分析中的研究效率和数据一致性.
自动化传统上劳动密集型的流程有助于更强大的科学发现.
这一协议为研究骨肌肉的研究人员提供了宝贵的资源.

关键词:

细胞生物学细胞生物学计算机科学计算机科学显微镜的使用方法

更多相关视频

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

Published on: March 28, 2017

Author Spotlight: Isolation of Long Muscle Fibers from Mouse Hindlimb Muscles for Studying Excitation-Contraction Coupling Across Fiber Types

Author Spotlight: Isolation of Long Muscle Fibers from Mouse Hindlimb Muscles for Studying Excitation-Contraction Coupling Across Fiber Types

Published on: December 1, 2023

相关概念视频

Classification of Skeletal Muscle Fibers

Classification of Skeletal Muscle Fibers

Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...