相关实验视频

Updated: Jun 3, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

设计一个优化的超紧的CRISPR/Cas12j-8系统,用于植物中高效的基因组编辑

Shasha Bai¹, Xingyu Cao¹, Lizhe Hu¹

¹Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, Inner Mongolia University, Hohhot, China.

Plant biotechnology journal

|January 12, 2025

概括

工程CRISPR Cas12j-8显示了植物基因组编辑效率的提高. 这种改进的系统可以实现精确的基编辑和核酶分裂,以改善作物.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
植物科学植物科学

背景情况:

Cas12j-8核酶是一个紧的V型CRISPR系统组件,由于其小小的尺寸和TTN PAM识别,它为作物基因组编辑提供了潜力.
然而,由于其在工厂中的编辑效率较低,其实际应用受到限制.

研究的目的:

为了提高植物Cas12j-8系统的基因组编辑效率.
为植物生物技术开发一个更强大,更通用的CRISPR工具.

主要方法:

对Cas12j-8核酶及其相关crRNA的合理工程.
在大豆和大米中测试工程系统的编辑活动.
开发使用工程 Cas12j-8 系统的细胞因子基编辑器.

主要成果:

改造的Cas12j-8和crRNA显著提高了植物的基因组编辑效率.
综合系统在大豆和大米领域表现出强有力的活动,编辑了以前无法访问的网站.
在某些情况下,编辑效率与SpCas9相匹配或超过,并且优于Cas12j-2变体.
开发的基础编辑器显示,在没有indels的基础编辑效率 (C到T) 中增加了5.36至6.85倍.

结论:

设计的超紧的CRISPR/Cas12j-8系统是植物基因组编辑的高效工具.
这种增强的系统促进了核酶介导的分裂和植物中精确的基编辑.
这些发现为使用CRISPR技术的先进作物改进策略铺平了道路.

关键词:

Cas12j-8的情况.基础编辑基础编辑这就是 crRNARNA.基因组编辑基因组编辑植物植物植物植物植物植物.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

What is Genetic Engineering?

What is Genetic Engineering?