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甲基二甲酸对斑马鱼胚胎和幼虫的发育和神经毒性影响

Weili Yang¹, Ruijing Li¹, Xingxue Yan¹

¹Henan Engineering Research Center of Zebrafish Models for Human Disease and Drug Screening, Henan Neurodevelopment Engineering Research Center for Children, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, China.

Aquatic toxicology (Amsterdam, Netherlands)

|January 12, 2025

概括

甲基甲酸盐 (DMP) 导致斑马鱼的发育神经毒性,降低了生存率和化率. 暴露于DMP还导致形和改变神经基因表达,表明环境风险.

科学领域:

环境毒理学环境毒理学
发育神经生物学的发展神经生物学
水生物质毒理学研究

背景情况:

甲基甲基甲酸 (DMP) 是一种广泛使用的可塑化剂,在环境中越来越多的存在.
人们担心它可能对生物产生不利影响,特别是在发育过程中.

研究的目的:

在斑马鱼胚胎和幼虫中研究二甲基甲酸盐 (DMP) 的发育神经毒性.
评估DMP对生存,化,形,行为,神经元细胞和基因表达的影响.

主要方法:

斑马鱼胚胎在受精后4至120小时内暴露在各种DMP度 (5-100 mg/L) 中.
分析了生存,化,形率和幼虫行为.
转基因斑马鱼 (Tg(elavl3:EGFP)) 和RT-qPCR被用来评估神经元细胞的影响和神经基因表达.

主要成果:

暴露于DMP降低了生存率和化率,并增加了形 (例如,脊椎曲,).
行为变化包括增加自发的尾巴卷曲和减少游泳距离.
DMP降低了神经元细胞光和下调了关键的神经系统标记基因 (gap43,mbp,α1-tubulin,syn2a).

结论:

丁甲基甲酸盐 (DMP) 在斑马鱼中表现出显著的发育神经毒性作用.
这些发现强调了与DMP暴露相关的生态和健康风险.
严格监管DMP的使用和环境释放对于保护水生生物至关重要.

关键词:

有关急性暴露的急性暴露行为行为行为.发展性毒性发展性毒性甲基二甲甲酸二甲基甲酸二甲基甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸二甲酸神经损伤神经损伤斑马鱼的幼虫斑马鱼的幼虫

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