基于构成依赖的MRM过渡和结构指示性化段 (CMTSES) 的LC-MS策略,用于分糖化和异构体分化
Wen Miao1, Na Li1, Jia-Qian Chen1
1Faculty of Chinese Medicine & State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
Analytica chimica acta
|January 12, 2025
概括
一种新的液体染色学三重四极质谱 (LC-QQQ-MS) 策略,CMTSES,分析了二糖和分化异构体. 该方法使用易于获得的参考资料,减少了结构分析中专门标准的需求.
科学领域:
- 碳水化合物化学 碳水化合物化学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 碳水化合物在生命科学中至关重要,但由于结构复杂而难以分析.
- 单体,甘氨酸键和配置中的异构体变异妨碍了准确的结构确定.
- 现有的方法通常需要专门的设备或众多标准来实现异构体的分化.
研究的目的:
- 开发一个全面的二糖化物分析和异构体分化策略.
- 克服复杂碳水化合物结构现有分析方法的局限性.
- 为了能够准确地分析分糖的结构,而减少对专业标准的依赖.
主要方法:
- 开发了一种基于构成依赖的MRM转换和结构指示性化段 (CMTSES) 的LC-QQQ-MS策略.
- 使用的前体和产物离子用于分糖化合物组成和通过MRM转换进行类型注释.
- 采用"位置参考" (曼诺,银,异) 来定义异构体识别的化段.
主要成果:
- 在CMTSES战略中,有效地区分了分糖的链接,组成和配置异构体.
- 在真实样本中成功识别出混合的同质和/或异质二糖同体.
- 获得了多种单体类型的脱糖体的综合结构信息,包括次要成分.
结论:
- 基于CMTSES的LC-MS策略提供了一种可靠的方法来分析二糖和异构体差异化.
- 使用"位置引用"可以通过减少对特定标准的需求来提高适用性.
- 对分子内键的进一步研究可以解决对其他二糖化合物的特征表征的局限性.
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