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通过酶自动化标记和生物直角反应预定目标多模式瘤成像

Yinxing Miao¹, Yuqi Wang¹, Yefeng Chen²

¹State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Road, Nanjing 210023, China.

Journal of the American Chemical Society

|January 13, 2025

概括

研究人员开发了E-SIM, 这是一种快速选择性标记癌细胞膜的新方法. 这种由酶触发的策略通过附加生物直角手柄来改善体内瘤成像和治疗.

科学领域:

生物结合化学
分子成像
癌症治疗方法

背景情况:

对瘤成像和治疗具有潜力.
目前的方法有缓慢的动力学,癌细胞选择性差,非目标效应和低于最佳的体内疗效.

研究的目的:

开发一种快速和选择性的瘤细胞膜标签策略,用于体内应用.
通过丰富癌细胞的生物直角报告器来实现预定目标的多模式成像和治疗.

主要方法:

引入了E-SIM (酶触发自动固定),使用一种性酸酶 (ALP) 响应的胺甲基 (QM) 前体 (P-TCO) 与一个转环基 (TCO) 组.
使用近距离标记,以快速,高密度的TCO处理到瘤细胞膜in vivo.
使用TCO手柄和含有四素 (Tz) 的记者之间的快速生物正交反应进行记者丰富.

主要成果:

已证明E-SIM能够在体内使用TCO手柄快速和选择性地标记瘤细胞膜.
在标记的瘤细胞膜上实现了各种大小的Tz载体的高效丰富.
通过使用Tz修饰的Renilla luciferase成功应用E-SIM进行肝癌转移的预定向生物发光成像,从而实现敏感的检测和手术指导.

结论:

在复杂的生物环境中,E-SIM是精确的瘤细胞标记的强大策略.
这种方法有助于针对多种瘤成像和治疗应用的各种报告者的预定丰富.
E-SIM提高了体内成像的灵敏度和准确性,为改善癌症检测和治疗指导提供了潜力.

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