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Updated: Jun 3, 2025

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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Published on: May 7, 2018

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工程基因和蛋白质开关用于调节谱系特异转录因子

Ana P Teixeira1, Nik Franko1, Martin Fussenegger1,2

  • 1Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.

Biotechnology and bioengineering
|January 13, 2025
PubMed
概括

研究人员使用病毒蛋白酶开发了新的基因和蛋白质开关,以精确控制人类多能干细胞 (hPSC) 中的细胞分化. 这些工具可以同时调节多个转录因子,用于再生医学和发育生物学研究.

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Lineage Commitment

Commitment is the  process whereby stem cells:
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Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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