基于分子的实验室测试比微观测试更准确,用于的识别
Isaac Singh1, Min-Kuang Lee1, Emily Kon2
1British Columbia Centre for Disease Control, 655 W 12th Ave, Vancouver, British Columbia, V5Z 4R4, Canada.
International journal for parasitology. Parasites and wildlife
|January 13, 2025
概括
该研究证实Ixodes pacificus是不列颠哥伦比亚省的主导,而不是Ixodes scapularis. 在BC发现的Ixodes scapularis与宿主出境地区的旅行有关,这凸显了监测的必要性.
科学领域:
- 兽医昆虫学 兽医昆虫学
- 医学昆虫学 医学昆虫学
- 分子诊断学 分子诊断
背景情况:
- 太平洋 (Ixodes pacificus) 是不列颠哥伦比亚省 (BC) 的主要莱姆病载体.
- Ixodes scapularis是加拿大东海岸的主要莱姆病载体.
- 最近的一份报告表明,BC地区存在Ixodes scapularis,这促使进一步调查.
研究的目的:
- 在BC疾病控制中心 (BCCDC) 公共卫生实验室评估微观标识的准确性.
- 将形态识别方法与分子识别技术进行比较.
- 为了确定Ixodes scapularis在BC的流行程度和起源.
主要方法:
- 微观识别BCCDC公共卫生实验室收到的209只.
- 使用实时PCR进行Ixodes scapularis查 (ITS2区域) 的分子鉴定.
- 通过桑格序列测定细胞染色体c氧化酶子单元1基因的物种确认.
主要成果:
- 太平洋 (Ixodes pacificus) 占识别的的74%,头皮 (Ixodes scapularis) 的比例为3.8%.
- 分子方法证实了显微镜识别的准确性.
- 在八个 Ixodes scapularis 主体中,有六个最近在 BC 外的旅行史.
结论:
- 太平洋 (Ixodes pacificus) 仍然是不列颠哥伦比亚省的主要物种.
- 在BC中识别的Ixodes scapularis可能与宿主旅行有关.
- 加强的监测至关重要,因为通过全球旅行促进了的迁徙.
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