相关实验视频
Updated: Jun 3, 2025

05:20
Zebrafish Model of Neuroblastoma Metastasis
Published on: March 14, 2021
2.7K
circ-ZEB1通过调节miR-491-5p/EIF5A轴来增强NSCLC的转移和扩散
Qi Wang1, Shengying Ling1, Jia Lv2
1Department of General Practice, Renji Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Analytical cellular pathology (Amsterdam)
|January 13, 2025
概括
循环RNA ZEB1 (circ-ZEB1) 通过调节miR-491-5p/EIF5A通路,促进非小细胞肺癌 (NSCLC) 的进展. 下调circ-ZEB1抑制NSCLC细胞增殖和迁移,表明其作为治疗点的潜力.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 在RNA生物学,RNA生物学.
背景情况:
- 循环RNAs (circRNAs) 与癌症进展有关.
- 在肝癌中,circ-ZEB1的表达异常.
- 在非小细胞肺癌 (NSCLC) 中circ-ZEB1的作用仍未明确.
研究的目的:
- 研究NSCLC中circ-ZEB1的表达和功能.
- 阐明circ-ZEB1在NSCLC进展中的潜在分子机制.
- 评估circ-ZEB1作为NSCLC的潜在治疗点.
主要方法:
- 使用光在位杂交 (FISH) 和RT-qPCR分析了circ-ZEB1的表达.
- 细胞增殖和迁移通过Transwell,EDU和CCK8试验进行评估.
- 进行了体内转移和瘤发生的测试,以及光酶记者测试,以确定下游目标.
主要成果:
- 在NSCLC组织和细胞中,circ-ZEB1的表达显著上调.
- 抑制circ-ZEB1的下调抑制NSCLC细胞的增殖和迁移,在体外和体内.
- circ-ZEB1直接针对EIF5A和miR-491-5p,EIF5A过度表达和miR-491-5p抑制可以逆转circ-ZEB1沉默对迁移的影响.
结论:
- 通过调节miR-491-5p/EIF5A轴,circ-ZEB1促进NSCLC恶性进展.
- circ-ZEB1代表了NSCLC的潜在诊断生物标志物和治疗标.
相关概念视频
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
The Tumor Microenvironment
6.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K

