模仿骨质状3D多孔密集微纤维丝纤维蛋白嵌入的聚乙烯醇) 脚手架,用于膜脊保护
Supaporn Sangkert1, Perumal Ramesh Kannan2,3, Jirut Meesane4
1Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai 90110, Thailand.
Regenerative biomaterials
|January 13, 2025
概括
一个由微纤维丝纤维素 (mSF) 和聚乙烯醇 (PVA) 制成的新型3D多孔支架有效地支持膜脊保护 (ARP). 5%的mSF脚手架显示出卓越的生物特性和骨质导体活性,用于骨再生.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 牙科植入物学 牙科植入物学
背景情况:
- 膜脊损失使牙植入物放置和稳定性变得复杂.
- 膜脊保护 (ARP) 对于维持骨和避免广泛的增生手术至关重要.
研究的目的:
- 开发和描述一种新的3D多孔脚手架,模仿状骨架模板,以保护膜脊 (ARP).
- 评估脚手架在骨再生方面的生物性能和骨质导电潜力.
主要方法:
- 使用微纤维丝纤维素 (mSF) 嵌入聚乙醇 (PVA) 通过冷解制造3D多孔脚手架.
- 使用3D形状测量和扫描电子显微镜进行形态,热,晶体和机械性能分析.
- 在体外评估骨质细胞粘附,增殖,活力,蛋白质合成,性酸酶活性和沉积.
- 在体内评估使用大鼠介质干细胞来模仿动态骨环境.
主要成果:
- 使用不同的mSF度 (1-7%) 的脚手架制造表明了可调节的特性.
- 增加的mSF含量增强了相互连接的孔径大小,热行为,胀和机械强度.
- 具有5%和7%mSF的脚手架显示降低了降解率.
- 5%的mSF支架表现出卓越的生物性能和骨质传导活性.
结论:
- 该mSF/PVA 3D多孔脚手架有效地模仿了骨模板,以保护膜脊.
- 5%的mSF度为骨再生提供了最佳的物理,机械和生物特性平衡.
- 这种支架具有显著的潜力,可以通过解决膜脊损失来提高牙科植入成功率.
相关概念视频
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Collagens are the Major Structural Proteins of ECM
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
Connective tissue proper includes loose...
Fibril-associated Collagen
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Matrix Proteoglycans and Glycoproteins
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Fibrous Proteins
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...


