相关实验视频
Updated: Jun 2, 2025

09:53
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
Published on: June 15, 2018
7.3K
微RNA miR-378-3p 是内皮细胞自和功能的一种新型调节剂
Shuhan Bu1, Jameela J Joseph1, Hien C Nguyen1,2
1Department of Medical Biophysics, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada.
Journal of molecular and cellular cardiology plus
|January 13, 2025
概括
微RNA miR-378-3p 负面调节内皮细胞自和功能. 它的抑制改善了内皮氧化合成酶 (eNOS) 水平和细胞迁移,而过度表达会损害这些过程.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 自是一种基本的细胞过程,用于降解和回收.
- 微RNAs (miRNAs) 是细胞功能的关键调节者,包括自和内皮健康.
- 在内皮细胞自和功能中miR-378-3p的特定作用需要阐明.
研究的目的:
- 研究miR-378-3p在调节内皮细胞自中的作用.
- 确定miR-378-3p对内皮功能的影响,包括增殖,迁移和管形成.
- 在内皮细胞中识别miR-378-3p的潜在分子标.
主要方法:
- 治疗内皮细胞以抑制或激活自,并测量miR-378-3p的表达.
- 获得和丧失功能的方法被用来操纵miR-378-3p水平.
- 评估了自的标记物 (LC3-II/LC3-I比),内皮功能 (增殖,迁移,管形成,eNOS表达) 和亡.
- 生物信息分析和实验验证用于确定miR-378-3p目标.
主要成果:
- 自调节反向影响了miR-378-3p的表达.
- 过度表达miR-378-3p损害了自和内皮功能,降低了LC3-II/LC3-I比率,eNOS表达和血管生成潜力,同时增加了增殖和迁移.
- 抑制miR-378-3p增强了eNOS表达并减少了扩散和迁移.
- 蛋白质二硫化异构酶家族A成员4 (PDIA-4) 被确定为miR-378-3p的直接目标.
结论:
- miR-378-3p作为内皮细胞自和功能的负调节剂.
- 这些发现揭示了涉及miR-378-3p和PDIA-4的新型表观遗传机制,用于控制内皮细胞的行为.
- 这项研究为内皮健康的调节和潜在的治疗点提供了新的见解.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K

