相关实验视频
Updated: Jun 2, 2025

07:24
A Protocol for the Production of KLRG1 Tetramer
Published on: January 12, 2010
9.9K
一个LGR二聚体的结构 - 糖蛋白激素受体的进化前身
Zhen Gong1, Shuobing Chen1, Ziao Fu1
1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
bioRxiv : the preprint server for biology
|January 13, 2025
概括
这项研究揭示了糖蛋白激素受体的二元结构,解释了这些受体如何作为对来调节生殖和新陈代谢. 这些发现澄清了激素作用和G蛋白信号传递机制.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 内分泌学 在内分泌学.
背景情况:
- 糖蛋白激素对生殖和新陈代谢至关重要,通过二次受体起作用.
- 人类葡萄糖蛋白激素受体在孤立的结构上是单体的,与功能上的二元化证据形成鲜明对比.
- 了解受体二分化是阐明激素作用和信号的关键.
研究的目的:
- 确定从线虫的同类糖蛋白激素受体的冷电子显微镜 (cryo-EM) 结构.
- 为这些受体的二维功能及其与激素的相互作用提供结构性见解.
- 解释功能性观测,如人体受体中的交换激活和负合作性.
主要方法:
- 电子显微镜 (cryo-EM) 用于确定线虫受体的结构.
- 与人类葡萄糖蛋白激素受体,特别是甲状腺刺激激素受体 (TSHR) 的结构比较.
- 在人类同类的已知功能数据的背景下对二维结构的分析.
主要成果:
- 冷-EM结构显示同源线虫受体的不对称二元,由其同源激素激活.
- 双面结构与人体甲状腺刺激激素受体 (TSHR) 有共同的特征.
- 这种二维模型解释了在人类受体中观察到的交易激活,负合作性和G蛋白切换.
结论:
- 这项研究提供了关于糖蛋白激素受体二元化的第一个结构证据.
- 二维结构解释了激素受体相互作用中的复杂功能现象.
- 这些发现推动了我们对参与生殖,新陈代谢和生长的激素信号通路的理解.
相关概念视频
Transducer Mechanism: G Protein–Coupled Receptors
1.8K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
GPCRs are also called heptahelical,...
1.8K
G Protein-coupled Receptors
11.2K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
11.2K
G-protein Coupled Receptors
115.4K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
115.4K
Transducer Mechanism: Enzyme-Linked Receptors
2.3K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.3K
Assembly of Signaling Complexes
5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Diversity of Antigen Receptors
501
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
501

