评估不同溶剂在细菌逆变异试验中的影响
Satyam N Patel1, Chetan K Kajavadara1, Rushikesh M Shukla1
1Department of Pharmacology and Toxicology, Zydus Research Centre, Zydus Lifesciences Limited, Ahmedabad, Gujarat, India.
Environmental and molecular mutagenesis
|January 13, 2025
概括
这项研究评估了用于细菌逆变异试验 (AMES试验) 的替代溶剂. 大多数测试的溶剂,不包括四二,被证明是兼容的,支持它们在突变性测试中的使用.
科学领域:
- 毒理学 毒理学 毒理学
- 基因毒性测试是指基因毒性测试.
- 化学安全评估 化学安全评估
背景情况:
- 细菌逆变异试验 (AMES试验) 对于评估化学变异性至关重要.
- 溶剂的选择对于达到足够的试剂溶解度和测试度至关重要.
- 对于细菌菌株 (例如,沙门氏菌 typhimurium,大肠杆菌 Escherichia WP2 uvrA) 寻找兼容的溶剂是一个挑战.
研究的目的:
- 评估各种替代溶剂对AMES测试的适用性.
- 为了确定溶剂与细菌菌株和测试条件的兼容性.
主要方法:
- 测试的溶剂包括N,N-二甲基形式胺,乙,乙二,乙烯酸,乙醇,二乙烯糖醇单甲基乙烯,甲醇,P-二氧化,四,和二甲基胺.
- 通过评估S9酶的抑制,细菌生长和细菌逆转殖民地数量来评估溶剂兼容性.
- 在高达100μL/盘的度下确定兼容性.
主要成果:
- 所有测试的溶剂,除了四基,与AMES测试兼容.
- 兼容的溶剂没有抑制S9酶,细菌生长或改变复原菌群数量.
- 在100μL/盘或更高的度下证明了兼容性.
结论:
- 几种替代溶剂适合用于细菌逆变异试验.
- 这些发现扩大了基因毒性测试的溶剂选择,改善了化学品安全评估.
相关概念视频
01:16In vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:28Antimicrobial Effectiveness
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...