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在怀孕期间使用草甘除草剂的压力会通过修改DNA甲基化来损害新生猪的肠道发育

Guangdong Bai^1,2, Hua Yuan², Mingming Cao²

¹College of Animal Science and Technology, Southwest University, Chongqing 400715, China.

Journal of agricultural and food chemistry

|January 13, 2025

概括

孕妇在怀孕期间接触草甘除草剂 (GBH) 会损害后代的肠道健康. 这发生在甲状腺中通过DNA低甲基化,影响发育和消化途径.

科学领域:

环境毒理学环境毒理学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学

背景情况:

草甘基除草剂 (GBH) 是常见的料污染物.
孕妇在怀孕期间接触毒素可以通过表观遗传机制导致胎儿的持久变化.
对于DNA甲基化对GBH诱导后代肠道损伤的作用尚不清楚.

研究的目的:

研究孕期草甘基除草剂 (GBH) 暴露对新生猪肠道DNA甲基化和健康的影响.
探索 GBH 诱导后代肠道损伤背后的表观遗传机制.

主要方法:

建立孕母猪暴露于GBH的模型.
对新生猪的肠功能和DNA甲基转移酶 (Dnmt1,Dnmt3b) 的mRNA表达的分析.
减少表示双硫酸盐测序 (RRBS) 用于全基因组DNA甲基化分析.
甲基组和转录组数据的综合分析.

主要成果:

孕期GBH暴露损害了肠道功能,并降低了Dnmt1和Dnmt3bmRNA表达在小猪内.
在RRBS分析中,发现了阴中显著的基因组低甲基化.
不同的甲基化区域在与肠道发育和食物消化相关的途径中得到了丰富.
综合性分析确定了23个具有逆DNA甲基化和mRNA表达相关性的基因,这表明了损伤机制.

结论:

孕妇在怀孕期间暴露于GBH会诱导后代阴中的DNA低甲基化,影响肠道的发育和功能.
表观遗传调节,特别是DNA甲基化,在母亲暴露于GBH后后代的肠道损伤中起着至关重要的作用.
研究结果强调,需要开发更安全的除草剂,以确保食品安全,保护人类和动物的健康.

关键词:

通过DNA甲基化.草甘基除草剂是一种基于草甘的除草剂.肠道的发育肠道的发育一个孩子的后代.怀孕的母猪怀孕了母猪

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