集成的微针和水凝生物传感器平台:朝着一个诊断设备收集和双模式传感麻病毒A29蛋白质的诊断设备
Yujian Liu1,2, Jiang Liu3, Yequn Chen4
1School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
Analytical chemistry
|January 13, 2025
概括
一个新的集成微针和水凝生物传感器 (IMHB) 平台,能够快速地在现场检测病毒 (MPXV). 这种双模式生物传感器可以直接从皮肤病变中提供灵敏的检测,帮助早期的传染病干预.
科学领域:
- 生物医学工程 生物医学工程
- 传染病诊断 传染病诊断 传染病诊断
- 生物传感器技术技术
背景情况:
- 猿疫情凸显了快速和敏感的病毒检测方法的需要.
- 微针 (MNs) 为便携式诊断设备提供最小侵入性采样.
研究的目的:
- 开发一个集成的微针和水凝生物传感器 (IMHB) 平台用于病毒 (MPXV) 检测.
- 为了使特定的提取和双模式 (电化学和色度) 检测MPXV直接从皮肤.
主要方法:
- 设计了一个IMHB平台,集成一个电子设备,一个采样的MN贴片和一个带有信号探头 (TMB) 的水凝贴片.
- 该平台测试了从病变皮肤和双模式检测中提取MPXV的特定提取.
- 一个控制区域用于比率分析和质量控制.
主要成果:
- 在IMHB平台成功地直接从损伤皮肤中提取MPXV.
- 双模式传感显示了MPXV A29蛋白的广泛检测范围 (10-1000 ng/mL).
- 检测极限为0.1632 ng/mL (电化学) 和0.3017 ng/mL (色度计).
结论:
- 开发的IMHB平台为快速的现场MPXV测定提供了一种新的方法.
- 这项技术显示了在传染病如麻疹的早期阶段及时干预的潜力.
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