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在老化加速小鼠中,联合运动诱导的Notch路径和肌肉质量的调节

Ana P Pinto¹, Ângelo Augusto J Sarni¹, Maria Eduarda A Tavares^2,3

¹School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Avenida Bandeirantes, 3900, Monte Alegre, Ribeirão Preto, São Paulo, 14040-907, Brazil.

Pflugers Archiv : European journal of physiology

|January 13, 2025

概括

介质素-10 (IL-10) 影响肌肉细胞中的Notch通路. 联合炼通过增强Notch路径来促进老年小鼠的肌肉再生,这表明对老年人有好处.

科学领域:

肌肉生理学肌肉生理学
蜂信号传输是如何进行的
衰老的研究研究.

背景情况:

诺奇信号通路对于骨肌肉功能至关重要,包括发育,再生和衰老.
介质蛋白-10 (IL-10) 在肌肉组织内的炎症和再生过程中发挥作用.

研究的目的:

为了研究IL-10和C2C12神经细胞中Notch通路之间的相互作用.
为了检查联合耐力和抵抗运动对老鼠骨肌的Notch和自途径的影响.
分析老年人骨肌中的Notch通路活性.

主要方法:

C2C12神经细胞被小干扰RNA感染,向IL-10 (siIL-10).
来自静坐和运动老化加速小鼠 (SAMP8和SAMR1) 的骨肌肉接受了组织学分析,RT-qPCR和免疫阻塞.
一个公共数据集被用来比较老年男性与年轻人的诺奇通路基因表达.

主要成果:

在神经细胞中IL-10的淘汰导致了诺奇通路基因和蛋白质表达的减少.
在SAMP8小鼠中,联合炼改善了肌肉纤维组织,运动协调,并增加了Notch2和Hes1mRNA水平.
与具有类似活动水平的年轻男性相比,老年男性的NOTCH2mRNA水平升高.

结论:

在骨肌细胞中,IL-10调节了Notch信号通路.
综合体育炼促进骨肌肉通过Notch路径在衰老中再生.
诺奇路径可能是与年龄相关的肌肉衰退的治疗目标.

关键词:

衰老的衰老衰老的衰老分子路径的分子路径.肌肉的发展肌肉的发展身体炼是一种体力炼.

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相关概念视频

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...