相关实验视频

Updated: Jun 2, 2025

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation

Published on: March 15, 2024

用Cas9和AAV修复模板进行基因组工程,成功和陷

M C Birling¹, Y Hérault^2,3, G Pavlovic²

¹CNRS, INSERM, CELPHEDIA, Institut Clinique de la Souris (ICS), Université de Strasbourg, Illkirch, PHENOMIN, France. birlingm@igbmc.fr.

Mammalian genome : official journal of the International Mammalian Genome Society

|January 13, 2025

概括

再组合腺相关病毒 (rAAV) 与CRISPR/Cas9相结合,增强基因组编辑,用于创建动物模型. 这种方法提高了产生精确突变的效率,克服了以前的交付挑战.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

克里斯普尔/Cas9是产生动物模型的强大工具.
为精确的突变提供捐赠者DNA,如敲入,仍然是低效的.
再组合腺相关病毒 (rAAV) 为捐赠者DNA输送提供了一个有前途的解决方案.

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...