相关实验视频

Updated: Jun 2, 2025

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

肝瘤细胞衍生的外体SNORD52通过激活JAK2 / STAT6通路来调解M2巨分极

Yaqiong Zhang¹, Bo Li², Wanhong Gu¹

¹Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital), No.999 Donghai Road, Taizhou, 318000, Zhejiang, China.

Discover oncology

|January 13, 2025

概括

来自肝瘤细胞的外体SNORD52通过JAK2/STAT6通路驱动M2巨分极,促进肝细胞癌 (HCC) 的进展. 这突出了HCC发展中的一种新机制.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
免疫学免疫学免疫学

背景情况:

肝细胞癌 (HCC) 的进展涉及小核RNA 52 (SNORD52) 的致癌作用.
外体SNORD52在HCC期间巨细胞极化中的功能在很大程度上是未知的.

研究的目的:

为了研究外体SNORD52在HCC中巨细胞极化中的作用.
阐明涉及JAK2/STAT6通路的潜在分子机制.

主要方法:

从肝瘤细胞中分离出外体.
评估M2巨细胞极化标志物通过流动细胞计和西部涂抹.
使用qRT-PCR对SNORD52表达的量化和对JAK2/STAT6通路蛋白的分析.

主要成果:

在肝瘤细胞的外体和HCK患者的血中,SNORD52是丰富的.
肝瘤细胞衍生的外体SNORD52促进M2巨细胞的两极分化,并激活JAK2/STAT6通路.
证实了巨细胞对SNORD52丰富的外体内化.

结论:

肝瘤细胞衍生出异位体SNORD52诱导M2巨细胞的极化.
激活JAK2/STAT6通路是外体SNORD52促进M2极化的机制.

关键词:

框 C/D 小核细胞RNA 52 52 异构体是什么? 异构体是什么? 外构体是什么?肝细胞癌是肝细胞癌.巨细胞是一个巨细胞.两极化的偏化

更多相关视频

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...