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在SELEX后,通过新乙化对氨酸胺基进行修改

Heidi Kähkölä¹, Muditha Herath¹, Pasi Virta¹

¹Department of Chemistry, University of Turku, Henrikinkatu 2, 20500 Turku, Finland. tuanlo@utu.fi.

Organic & biomolecular chemistry

|January 13, 2025

概括

一种新的方法使用动态化学修改了SELEX之后的aptamers. 修改后的阿巴体对因表现出独特的反应,包括二元化和键裂解,从而使新的阿巴体应用成为可能.

科学领域:

化学生物学化学生物学
生物化学生物化学
核酸化学的核酸化学

背景情况:

SELEX (通过指数式丰富对联体的系统进化) 是一种强大的体发现技术.
在SELEX后的修改允许对aptamer的特性和功能进行微调.
动态组合化学为分子识别和传感器开发提供了新的方法.

研究的目的:

引入一种基于新乙化的方法,用于SELEX后的胺基修饰.
为了研究在因的存在和缺席下修改的体的行为.
探索动态组合化学在aptamer功能化中的潜力.

主要方法:

通过添加 (2R,3S) -4- ((甲氧氨基) 丁-1,2,3-三醇残留物来合成修饰的阿普坦基基架.
化阿普坦与化物混合物和来形成N-甲-1,3-oxazinane (MOANA) 核酸相似物.
用UHPLC-MS分析识别反应性化物和ITC以确定结合亲和关系.

主要成果:

两种甲基化物,甲基-4-甲基酸和3-酸甲,显示了与修改的阿普他默支架的差异反应性.
在因结合后,3-基甲衍生型的阿胺异常二元化.
氨酸结合诱导N-O键裂变在另一个aptamer的修饰残留物中.

结论:

新乙化为SELEX后的胺基修饰提供了一个多功能平台.
动态组合化学可以用来创建具有独特因响应行为的体.
这些发现为开发新型适应传感器和分子探测器开辟了道路.

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