在庇护所猫中检测到SARS-CoV-2和可能的变种
Ogi Okwumabua1, Nancy Bradley-Siemens2, Catherine Cruz1
1Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, United States of America.
PloS one
|January 13, 2025
概括
物猫可以携带SARS-CoV-2 (导致COVID-19的病毒),可能将其传播给人类. 基因分析揭示了猫类样本中的突变,这些突变妨碍了准确的病毒检测.
科学领域:
- 兽医病毒学 兽医病毒学
- 动物传播疾病 动物传播疾病
- 分子诊断学 分子诊断
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 导致全球大流行,影响人类和动物的健康.
- 该病毒已在各种动物物种中检测到,这引发了人们对其在One Health倡议中的作用的担忧.
- 了解猫等伴侣动物的SARS-CoV-2储存库对于公共卫生监测至关重要.
研究的目的:
- 为了调查庇护所猫的SARS-CoV-2感染的患病率.
- 评估标准诊断方法在猫类样本中检测SARS-CoV-2的有效性.
- 在猫种群中识别SARS-CoV-2的潜在遗传变异.
主要方法:
- 收集了350只庇护所猫的结膜,喉拭子和血清样本.
- 使用实时逆转录聚合酶连锁反应 (rRT-PCR) 检测SARS-CoV-2,针对N1和N2基因.
- 利用SARS-CoV-2代理病毒中和测试 (sVNT) 和序列分析进行确认和遗传特征.
主要成果:
- 在使用标准N1/N2探针的猫头抽样中检测到SARS-CoV-2的低检测率 (2/350阳性,145/350不确定的).
- 在猫类SARS-CoV-2RNA的N2探针结合区域中发现了基对删除/替代.
- 经过修改的N2探针检测到96.9%的先前N2阴性样本中检测到SARS-CoV-2;除了一个血清样本外,所有血清样本都是抗体阴性.
结论:
- 由于遗传变异,标准SARS-CoV-2诊断试验可能对猫不那么敏感.
- 收容所的猫可以携带SARS-CoV-2,这可能会造成进一步传播和人类感染的潜在风险.
- 需要进一步的研究来描述与SARS-CoV-2相关的猫类潜在的新型冠状病毒.
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