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在PON2中断后,对口腔状细胞癌细胞系深化西斯普拉丁的敏感性:FTIRM调查

Alessia Belloni¹, Roberto Campagna², Valentina Notarstefano¹

¹Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche Via Brecce Bianche 60131 Ancona, Italy.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|January 13, 2025

概括

沉默酶偏氧酶-2 (PON2) 增强了对口腔状细胞癌 (OSCC) 的思普拉丁化疗疗效果. 这种方法克服了化学抵抗,为改善癌症治疗结果提供了一个有希望的策略.

科学领域:

生物化学生化学
在瘤学瘤学.
频谱学是一种光谱学.

背景情况:

西斯是口腔状细胞癌 (OSCC) 的关键化疗剂.
由于攻击性和化学抵抗性,OSCC的预后不佳.
氧酶-2 (PON2) 是一种酶,可以在癌细胞中赋予西斯普拉丁耐药性.

研究的目的:

调查沉默PON2是否可以提高OSCC中西斯的疗效.
分析PON2沉默OSCC细胞中cisplatin治疗后的依赖时间的细胞变化.
识别可表明化疗反应的光谱标志物.

主要方法:

在HOC621和HSC-3的OSCC细胞线上利用福利埃变换红外显微镜 (FTIR) 进行了PON2淘汰.
对光谱数据进行多变量和单变量统计分析.
将FTIR结果与MTT试验数据进行比较,以评估细胞活力和生长.

主要成果:

在两种细胞系中,PON2敲击证明了对西斯丁疗效的积极影响.
在PON2沉默和西斯治疗后,与HSC-3细胞相比,HOC621细胞的生长减少.
确定了特定的光谱标记物 (A1172/A_TOT,A1053/A_TOT,A967/A1080,A992/A_TOT),表明核酸和碳水化合物的细胞变化,在HOC621细胞中更为明显.

结论:

在OSCC中,PON2沉默是一种可行的策略,可以克服西斯普拉丁耐药性.
FTIR显微镜可识别与化疗反应相关的生化变化.
准PON2可能会改善OSCC患者的治疗结果.

关键词:

西斯普拉丁 (Cisplatin) 是一种里埃转换红外显微光谱法红外显微光谱法在 HOC621 细胞系中.在HSC-3细胞系中.多变量分析多变量分析.口腔状细胞癌的癌症帕洛克松酶-2 (PON2) 是一种

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