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基于综合生物信息学和实验分析的基础上,确定中与二硫化相关的生物标志物

Sijun Li¹, Lanfeng Sun¹, Hongmi Huang¹

¹Department of Neurology, the First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, Guangxi, China.

Neurobiology of disease

|January 13, 2025

概括

这项研究确定了9个关键分子参与了与相关的细胞死亡过程 - - 脱硫脱. 结果揭示了特定的分子变化和用于预测的机器学习模型,突出显示SLC7A11作为潜在的生物标志物.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

(EP) 是一种脑部疾病,其特征是经常性发作,细胞死亡与其病变有关.
失硫性症是一种新的细胞死亡机制,可能与有关,但其确切作用尚不清楚.
识别的基础分子机制对于开发向疗法至关重要.

研究的目的:

调查与相关的分子 (DRM) 在病发病的作用.
在中使用基因表达数据集识别差异表达的DRMs (DE-DRMs).
开发一种基于DE-DRM表达的预测模型.

主要方法:

对基因表达综合 (GEO) 数据集 (GSE33000,GSE63808,GSE143272) 的分析,以确定DE-DRMs.
基于患者DE-DRM表达的相关性分析和分子聚类.
机器学习模型开发 (随机森林) 和使用独立数据集进行验证.
在体外和体外发作模型中对DE-DRM表达和相互作用进行实验验证.

主要成果:

确定了九个DE-DRM:GYS1,SLC3A2,SLC7A11,NDUFS1,OXSM,LRPPRC,NDUFA11,NUBPL,以及NCKAP1.1 这些都是DE-DRM.
在NDUFS1-NDUFA11-NUBPL-LRPPRC和SLC3A2-SLC7A11.11之间发现了特定的相互作用.
随机森林模型证明了的最佳预测性能.
GNAQ与氨酸抗性有关.
实验验证证证实SLC7A11上调,SLC3A2-SLC7A11复合体增加,NDUFS1-NDUFA11复合体减少.

结论:

这项研究提供了新的证据,将dissulfidptosis与病原发生联系起来.
建议SLC7A11作为的特定DRM.
在SLC7A11-SLC3A2和NDUFS1-NDUFA11蛋白质复合体中的改变与有关.

关键词:

与二硫化相关的分子.是一种病.蛋白质复合体是一种蛋白质复合体.在 SLC7A1111 中.发作在发作.

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