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Updated: Jun 2, 2025

Robust 3D DNA FISH Using Directly Labeled Probes
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两级空间定位近距离标记用于跨生物层次测量和操纵.

Liusheng Chen1, Yiran Li1, Yuna Guo2

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.

Angewandte Chemie (International ed. in English)
|January 13, 2025
PubMed
概括

这项研究引入了一个双层近距离标记 (P2L) 平台,用于精确的分子映射复杂的生物系统. P2L能够实现先进的细胞分辨和向细胞组装,为细胞相互作用提供新的见解.

关键词:
细胞组装组件 细胞组装组酶级联是一种酶级联.逻辑计算计算的逻辑计算靠近标签的标签.两个层次的空间定位定位.

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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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