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Updated: Jun 2, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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转录因子MAX的突变通过改变折叠和结合途径,以全雌性方式增加DNA选择性.

Renee Hastings1, Arjun K Aditham2,3, Nicole DelRosso1

  • 1Biophysics Program, Stanford University, Stanford, CA, USA.

Nature communications
|January 13, 2025
PubMed
概括

蛋白质工程旨在通过了解连接体歧视来增强蛋白质选择性. 转录因子MAX的突变通过蛋白质构成的全调节改变了DNA选择性,而不是直接与DNA接触.

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