微生物在竞争生活方式中的生长促进了土壤微生物群中ARG的增加:基于遗传特征的见解
Zishu Liu1,2,3, Xiangwu Yao2, Chengyi Chen2
1Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Microbiome
|January 13, 2025
概括
抗生素耐药性基因 (ARG) 在低抗生素压力下与具有竞争力的土壤微生物相增加. 这些微生物生长得更快,拥有更多的ARG,并增强了资源获取,推动了耐药性传播.
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 生态生态学 生态生态学
背景情况:
- 由于环境的抗生素压力,抗生素耐药性基因 (ARG) 正在增加.
- 微生物生长和ARG丰富的平衡机制尚不清楚.
- 研究了土壤微生物群对不同度的氧基环素 (OTC) 的反应.
研究的目的:
- 了解土壤微生物如何平衡生长与抗生素耐药性基因 (ARG) 丰富.
- 在OTC压力下揭示ARG,细菌种类和功能基因之间的关联.
- 探索ARG在土壤中传播的生态驱动因素.
主要方法:
- 在环境到临床度下使用氧化四环素 (OTC) 进行微观种植.
- 射击枪转基因组学用于抵抗体和功能基因分析.
- 细菌生长量化和基于特征的土壤微生物群的评估.
主要成果:
- ARG的丰富度和丰富度与OTC度有非单调的变化,在中间水平 (0.1-0.5 mg/l) 达到峰值.
- Pseudomonadaceae 的生长与 ARG 的增加相关,由染色体携带的多药性耐药性基因介导.
- 增强的资源获取,增长收益率,能源生产和简化代谢途径推动了在亚抑制性OTC压力下ARG的丰富性.
结论:
- 具有更快增长和更高ARG负载的竞争性土壤微生物是通过亚抑制性抗生素度和营养稀缺性来选择的.
- 基于特征的评估为ARG生态和传播提供了洞察力.
- 土壤抗体的变化与微生物群的生活方式的变化有关,支持对ARG控制的竞争排除.
相关概念视频
Antibiotic Selection
52.2K
Overview
52.2K
Bacterial Transformation
55.1K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
55.1K
Genomic DNA in Prokaryotes
43.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.4K
Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Genome Size and the Evolution of New Genes
7.9K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
7.9K
Microorganisms in Medicine and Therapeutics
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.


