被可可病原体Moniliophthora perniciosa感染的番茄植物的根部发育受到糖的有限可用性影响
Daniele Paschoal1,2, Laura Cazetta1,2, João V O Mendes1,2
1Centro de Energia Nuclear na Agricultura, Universidade de São Paulo (USP), Piracicaba, São Paulo, Brazil.
Plant, cell & environment
|January 14, 2025
概括
巧克力中的魔女扫病是由Moniliophthora perniciosa引起的,通过在感染部位创建糖槽来损害番茄根的生长,而不是激素失衡. 细胞因素在这种光吸收干扰中发挥着关键作用.
科学领域:
- 植物病理学 植物病理学
- 分子植物科学 分子植物科学
- 植物生理学 植物生理学
背景情况:
- 蒙尼里奥弗托拉 (Moniliophthora perniciosa) 在可可 (Theobroma cacao) 中引起巫婆扫病.
- 该病原体还感染番茄 (Solanum lycopersicum) "微型米" (MT) 品种,导致茎胀和根生物量减少.
- 该研究调查了受感染的MT植物根生长受损的原因,探索激素失衡与水槽竞争.
研究的目的:
- 为了确定受感染的MT植物根生长受损是否是由于荷尔蒙失衡或水槽竞争.
- 阐明激素,特别是auxin和cytokinin在根生长表型中的作用.
- 了解光同化物分配和糖信号对根部发育障碍的贡献.
主要方法:
- "微型米"番茄植物感染了Moniliophthora perniciosa的感染.
- 根样本的RNA-seq分析以确定差异表达的基因.
- 激素水平分析在根.
- 使用对辅酶敏感的突变菌和缺乏细胞因素 (35S::AtCKX2) 的转基因菌株.
- 13CO2追踪以评估光同化物分配.
- 外源的糖糖应用于根.
主要成果:
- 受感染的MT植物显示根生物量减少,主要影响横向根,与茎胀相吻合.
- 激素分析没有显示出根激素水平或激素代谢基因表达的显著变化.
- 一种低细胞因素的转基因系 (35S::AtCKX2) 呈现出较少的症状,没有根生长障碍.
- 在受感染的MT植物的根部显著降低了C,这表明光吸收物运输受损.
- 外源糖部分恢复了受感染植物的根生长.
结论:
- 感染MT植物的侧根发育受损是由于糖信号和光吸收物供应中断.
- 通过细胞因素介导,在感染部位建立强大的糖槽,有助于在根部减少代谢物的可用性.
- 魔女扫病病原体破坏了植物资源分配,通过糖槽竞争而不是直接激素失衡,影响了根植的生长.
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