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High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
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在脂质纳米反应器中的基于分子识别驱动的动态共价化学的多巴胺光人工受体

Bohdan Kozibroda1,2, Jean-Marie Lehn2, Andrey S Klymchenko1

  • 1UMR 7021 CNRS, Laboratoire de Bioimagerie et Pathologies, ITI SysChem-Chimie des Systèmes Complexes, Université de Strasbourg, 74 route du, Rhin, 67401, Illkirch, France.

Angewandte Chemie (International ed. in English)
|January 14, 2025
PubMed
概括

化学家们开发了一种新的人工受体来检测多巴胺. 这种动态系统在脂质纳米反应器中使用光,在生物样本中感知敏感和选择性的神经递质.

关键词:
动态的人工受体 动态的人工受体动态的 imine 化学光探测器的光探测器分子识别分子识别神经递质检测神经递质检测

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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