相关实验视频

Updated: Jun 2, 2025

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

hsa_circ_0015388 在克罗恩氏病中减少巨衍生反应性氧物种

Yuya Sugiyama¹, Hiroaki Konishi², Tatsuya Dokoshi³

¹Division of Metabolism and Biosystemic Science, Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Midorigaoka-Higashi 2-1-1-1, Asahikawa Hokkaido, 078-8510, Japan.

Inflammatory bowel diseases

|January 14, 2025

概括

循环RNAs (circRNAs) 与克罗恩病 (CD) 的病原发生有关. 这项研究确定了hsa_circ_0015388作为CD中的关键circRNA,它抑制了巨细胞中反应性氧物种 (ROS) 的产生.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

克罗恩氏病 (CD) 是一种复杂的炎症性肠病,原因不明.
循环RNAs (circRNAs) 正在成为各种疾病病理中的重要参与者.
环RNAs在CD病变发生中的特定作用在很大程度上仍未被探索.

研究的目的:

在克罗恩病患者中研究circRNAs的差异表达.
为了确定参与CD.内免疫反应的特定circRNAs.
阐明一个关键的circRNA在CD中巨介导炎症中的功能作用.

主要方法:

来自CD和非CD个体的末端结核活检的RNA测序.
使用定量逆转录酶-聚合酶链反应 (qRT-PCR) 验证差异表达的circRNAs.
在具有circRNA下调的巨类THP-1细胞中进行功能分析,评估活性氧物种 (ROS) 生产,细胞因子表达和细胞迁移.

主要成果:

在CD和非CD组之间发现31个circRNAs显著失调.
Hsa_circ_0015388在CD患者中显著上调,并在THP-1细胞中表达.
在THP-1细胞中对hsa_circ_0015388的降低调节增加了ROS的产生,并改变了先天免疫路径,这被TNFSF10抑制所缓解.

结论:

Hsa_circ_0015388 在克罗恩病中失调.
Hsa_circ_0015388在巨细胞中表达,并通过TNFSF10通路负面调节ROS的产生.
这项研究强调了hsa_circ_0015388作为通过调节巨细胞ROS生产来调节CD的潜在治疗标.

关键词:

克罗恩氏病是什么克罗恩氏病是什么圆形的RNARNA是一个圆形的RNA.巨细胞是什么?巨细胞是什么?有活性氧物种的反应性氧物种.

更多相关视频

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Published on: March 7, 2019

相关概念视频

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...