通过结结驱动的自适应性协同活体成为原细胞
Donglei Wang1, Peiyu Zhang1, Qi-Zhi Zhong1
1Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
ACS applied materials & interfaces
|January 14, 2025
概括
由聚乙烯糖醇和酸形成的由结合驱动的协体提供稳定,可调节的原细胞. 这些适应性协生物模仿细胞功能,并且在高盐条件下强壮.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 合成生物学 合成生物学
背景情况:
- 通过液-液相分离 (LLPS) 的凝聚是人工原细胞和模仿无膜有机体的关键.
- 传统的协体依赖于静电相互作用,限制了它们在高离子强度 (>0.1M) 的稳定性.
- 需要新的驱动力和组件,以使合成协体与自然器官的强度相匹配.
研究的目的:
- 为了开发以结为驱动的适应性协体.
- 为了研究这些新型协虫的液态相分离 (LLPS) 行为.
- 探索它们作为原细胞和治疗交付中的潜力.
主要方法:
- 聚乙烯糖醇 (PEG) 和酸 (TA) 的复合,形成协体.
- 通过调整PEG和TA度和质量比,调整协同合性质.
- 评估高离子强度溶液 (高达1M) 中的协体稳定性.
- 评估原细胞模仿细胞行为,如新陈代谢,细胞和膜融合.
主要成果:
- 从PEG和TA成功形成了由结合驱动的自适应性协体.
- 证明可调节的LLPS行为,控制同体大小 (70nm到10μm) 和形态 (颗粒,空心囊).
- 在离子度高达 1 M 的情况下达到高稳定性,超过了传统的协体.
- 展示原细胞的能力,包括营养吸收,细胞和膜融合.
结论:
- 开发了一个平台,用于合理设计由结合驱动的协体.
- 在适应性协动物中实现可控制的尺寸和形态.
- 突出了由于增强的稳定性和功能,在原细胞构造和治疗中潜在的应用.
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