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关于IR-MALDESI质谱仪对PFAS进行成像的可行性

Allen Martin¹, Alena N Joignant¹, Matt Farrell²

¹FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.

Journal of mass spectrometry : JMS

|January 14, 2025

概括

这项研究引入了红外矩阵辅助激光脱吸电喷电离 (IR-MALDESI) 技术,用于检测组织中的 perfluoroalkyl 和 polyfluoroalkyl 物质 (PFAS). 虽然IR-MALDESI显示出希望,但其检测极限不足以分析斑马鱼组织中的PFAS生物积累.

科学领域:

环境化学环境化学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

perfluoroalkyl和 polyfluoroalkyl物质 (PFAS) 是广泛的环境污染物,这是由于自20世纪40年代以来广泛的工业使用.
测量水生生物体中的PFAS生物度对于了解它们的环境持久性和影响至关重要.
传统的LC-MS/MS方法量化了PFAS总量,但缺乏生物体内的空间分布数据.

研究的目的:

通过使用红外矩阵辅助激光脱电喷雾电离 (IR-MALDESI) 来评估几个PFAS的检测极限 (LOD).
为了比较冰矩阵与TMGN冰矩阵的疗效,用于PFAS的IR-MALDESI分析.
评估斑马鱼中特定PFAS的生物积累,并与IR-MALDESI能力进行比较.

主要方法:

使用IR-MALDESI测定小鼠肝脏组织上的PFAS LOD,使用冰和TMGN-冰矩阵.
perfluorooctane 硫酸 (PFOS) 的 LOD 确定为 0.16 fmol/voxel.
暴露于PFOA,PFOS和PFHxS的斑马鱼在提取后使用LC-MS/MS分析生物积累.

主要成果:

单独的冰矩阵给出了比IR-MALDESI PFAS分析的TMGN-冰组合更高的响应.
在斑马鱼中,PFOS的生物积累量最高,为731.9μg/kg.
对暴露于PFAS的斑马鱼的IR-MALDESI分析未能生成图像,因为PFAS水平低于检测极限.

结论:

在IR-MALDESI中,PFAS检测有潜力,PFOS的LOD值得注意.
在这种情况下,冰矩阵比TMGN-冰矩阵更有效用于IR-MALDESI PFAS分析.
目前的IR-MALDESI检测极限不足以在斑马鱼组织等复杂的生物样本中可视化低水平的PFAS生物积累.

关键词:

在 IR-MALDESI 找马尔代斯在 LCMS/MS 中使用.在PFAS中,有很多方法.质谱仪成像成像质谱仪成像成像斑马鱼是一种斑马鱼.

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