纳米孔RNA的直接测序确定了m⁶对于果中对Alternaria alternata的索尔比托尔受控耐药性至关重要的一种修饰

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Zhihua Song¹, Qing Yang¹, Biying Dong¹

¹State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China; The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China; Ecological Observation and Research Station of Heilongjiang Sanjiang Plain Wetlands, National Forestry and Grassland Administration, Beijing Forestry University, Beijing 100083, China.

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概括

索尔比托尔通过调节基因表达来信号果对Alternaria alternata的抗性. 通过MdVIR1和MdVIR2甲基转移酶对关键mRNA的N6-甲基氨酸 (m6A) 修饰对于这种索尔比托尔控制的防御机制至关重要.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物化学生物化学

背景情况:

索尔比托是罗萨属果树中一个关键的碳水化合物,它调节了对Alternaria alternata的抵抗力.
在sorbitol介导的植物防御中,N6-甲基亚丁素 (m6A) mRNA修饰的作用在很大程度上是未知的.

研究的目的:

调查m6A修饰对果中索尔比托尔控制的Alternaria耐药性的参与.
为了确定涉及该途径的特定甲基转移酶和向基因.

主要方法:

在变化的比醇水平下对m6A修饰的全转录组分析.
对索尔比托和病原体注射有反应的甲基转移酶 (MdVIR1,MdVIR2) 的鉴定和表征.
分析m6A修改对目标mRNA稳定性和翻译效率的影响.

主要成果:

减少索尔比托尔合成导致果叶转录中的m6A修饰的全球减少.
两种新的甲基转移酶,MdVIR1和MdVIR2,被确定为抵抗的关键调节剂.
MdVIR1和MdVIR2向MdWRKY79和MdNLR16mRNAs,通过m6A修改稳定它们并增强翻译.

结论:

由MdVIR1和MdVIR2介导的m6A修饰对于果中索比特醇诱导的对Alternaria alternata的耐药性至关重要.
这一途径突出了一个新的分子机制,将碳水化合物代谢与植物免疫联系起来.

关键词:

另一个是Alternaria alternata.果果果果是什么意思直接进行RNA测序.表观遗传修饰是一种表观遗传修饰.m(6) A6) 一个索尔比托尔 (Sorbitol) 是一种类型的醇.

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